anti γ cop  (Santa Cruz Biotechnology)

Journal: Science Advances

Article Title: The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection

doi: 10.1126/sciadv.adz6792

Figure Lengend Snippet: ( A ) HeLa S3 cells were uninfected or infected with HPV16.L2F (MOI, ~100). PLA (green) for IPO7 and FLAG (to detect L2) was performed at 24 hpi; nuclei were stained with DAPI (blue). Similar results were obtained in two independent experiments. Scale bar, 10 μm. ( B ) PLA fluorescence intensity per cell (>100 cells) as in (A), shown as individual values, means, and SDs, with statistical significance determined by one-way ANOVA. **** P < 0.0001. ( C ) Whole-cell extracts from uninfected or HPV16.L2F-infected (MOI, ~1) HeLa cells were collected at the indicated time points. A portion of the resulting extracts (input) was analyzed by immunoblotting for FLAG (to detect L2) and IPO7. The remaining extracts were subjected to IP with an anti-IPO7 antibody or control IgG and analyzed by immunoblotting for FLAG and IPO7. ( D ) HeLa cells were pretreated with 9 μM RO3306 or DMSO as a control for 24 hours and then uninfected or infected with HPV16.L2F (MOI, ~1), with RO3306 or DMSO maintained in the medium. At 24 hpi, cells were lysed and analyzed in (C). ( E ) HeLa cells treated with 10 nM Scr or a mixture of 5 nM COPA and 5 nM COPG1 ( COPA/G1 ) siRNA were uninfected or infected with HPV16.L2F PsV (MOI, ~1). At 24 hpi, cells were lysed, and a portion of the extracts (input) was immunoblotted for FLAG, IPO7, γ-COP, or β-actin (as a loading control). The remaining extracts were subjected to IP with an anti-IPO7 antibody or a control IgG and analyzed as in (C). ( F ) As in (E), except cells were transfected with 10 nM Scr or IPO7 siRNA #2 before infection. Samples were subjected to IP with an anti–γ-COP antibody or control IgG and analyzed as in (E).

Article Snippet: γ-COP , Rabbit , 12393-1-AP; Proteintech , WB and IP.

Techniques: Infection, Staining, Fluorescence, Western Blot, Control, Transfection

Journal: The EMBO Journal

Article Title: COPI – TRAPPII activates Rab18 and regulates its lipid droplet association

doi: 10.15252/embj.201694866

Figure Lengend Snippet: Control siRNA depletion (siCtrl) was conducted with siRNA oligos specific to a firefly luciferase sequence; siCOPB = depletion of β‐COP; siRab18 = depletion of Rab18. C9C10KO = HEK293T cells with TRAPPC9 and TRAPPC10 genes deleted. N = 3, error bars = SD.

Article Snippet: Mouse monoclonal antibodies against γ‐COP (sc‐271362), TRAPPC10 (clone RR‐18, sc‐101259), ADRP (sc‐377229), and LAMP2 (sc‐18822) were purchased from Santa Cruz Biotechnology.

Techniques: Control, Luciferase, Sequencing

Journal: The EMBO Journal

Article Title: COPI – TRAPPII activates Rab18 and regulates its lipid droplet association

doi: 10.15252/embj.201694866

Figure Lengend Snippet: Huh‐7 cells were first serum‐starved and then incubated with oleic acid for the indicated time before staining with Bodipy 493/503, TRAPPC9, Rab18, and γ‐COP.

Article Snippet: Mouse monoclonal antibodies against γ‐COP (sc‐271362), TRAPPC10 (clone RR‐18, sc‐101259), ADRP (sc‐377229), and LAMP2 (sc‐18822) were purchased from Santa Cruz Biotechnology.

Techniques: Incubation, Staining

Journal: The EMBO Journal

Article Title: COPI – TRAPPII activates Rab18 and regulates its lipid droplet association

doi: 10.15252/embj.201694866

Figure Lengend Snippet: Huh‐7 cells were first serum‐starved and then incubated with oleic acid for the indicated time before staining with Bodipy 493/503, Rab18, and γ‐COP. In the merged images, Bodipy 493/503 was pseudocolored in green, Rab18 in red, and γ‐COP in blue. In the colocalization between Rab18 and γ‐COP on the right side, the γ‐COP signal was re‐colored to green for easy visualization. Scale bar = 10 μm.

Article Snippet: Mouse monoclonal antibodies against γ‐COP (sc‐271362), TRAPPC10 (clone RR‐18, sc‐101259), ADRP (sc‐377229), and LAMP2 (sc‐18822) were purchased from Santa Cruz Biotechnology.

Techniques: Incubation, Staining